| 1. Identity statement | |
| Reference Type | Journal Article |
| Site | mtc-m21b.sid.inpe.br |
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S |
| Identifier | 8JMKD3MGP5W34M/3GD3GLQ |
| Repository | sid.inpe.br/mtc-m21b/2014/05.30.02.23.55 (restricted access) |
| Last Update | 2014:08.01.18.30.28 (UTC) administrator |
| Metadata Repository | sid.inpe.br/mtc-m21b/2014/05.30.02.23.56 |
| Metadata Last Update | 2018:06.04.03.04.16 (UTC) administrator |
| DOI | 10.1016/j.msec.2014.03.002 |
| ISSN | 0928-4931 |
| Label | scopus 2014-05 HollandaLoLaBeCoZa:2014:GrCaNa |
| Citation Key | HollandaLoLaBeCoZa:2014:GrCaNa |
| Title | Graphene and carbon nanotube nanocomposite for gene transfection  |
| Project | CNPq (202439/2012-7); FAPESP (2011/17195-3) and (2011/17877-7). |
| Year | 2014 |
| Month | June |
| Access Date | 2024, Apr. 19 |
| Type of Work | journal article |
| Secondary Type | PRE PI |
| Number of Files | 1 |
| Size | 2245 KiB |
| 2. Context | |
| Author | 1 Hollanda, L. M. 2 Lobo, A. O. 3 Lancellotti, M. 4 Berni, E. 5 Corat, Evaldo José 6 Zanin, Hudson |
| Resume Identifier | 1 2 3 4 5 8JMKD3MGP5W/3C9JH33 |
| Group | 1 2 3 4 5 LAS-CTE-INPE-MCTI-GOV-BR 6 LAS-CTE-INPE-MCTI-GOV-BR |
| Affiliation | 1 UNICAMP 2 UNIVAP 3 UNICAMP 4 UNICAMP 5 Instituto Nacional de Pesquisas Espaciais (INPE) 6 Instituto Nacional de Pesquisas Espaciais (INPE) |
| Author e-Mail Address | 1 2 3 4 5 corat@las.inpe.br 6 hudsonzanin@gmail.com |
| e-Mail Address | marcelo.pazos@inpe.br |
| Journal | Materials Science and Engineering C |
| Volume | 39 |
| Number | 1 |
| Pages | 288-298 |
| Secondary Mark | A1_ADMINISTRAÇÃO,_CIÊNCIAS_CONTÁBEIS_E_TURISMO A1_INTERDISCIPLINAR A1_ENGENHARIAS_II A2_CIÊNCIA_DE_ALIMENTOS A2_MEDICINA_VETERINÁRIA A2_MATERIAIS B1_MEDICINA_I B1_ENGENHARIAS_IV B1_FARMÁCIA B1_ARQUITETURA_E_URBANISMO B1_BIODIVERSIDADE B1_CIÊNCIAS_BIOLÓGICAS_II B1_QUÍMICA B1_ODONTOLOGIA B1_CIÊNCIAS_BIOLÓGICAS_I B1_BIOTECNOLOGIA B1_MEDICINA_II B1_ENGENHARIAS_III B1_CIÊNCIAS_BIOLÓGICAS_III B2_ASTRONOMIA_/_FÍSICA C_ZOOTECNIA_/_RECURSOS_PESQUEIROS C_CIÊNCIAS_AGRÁRIAS_I |
| History (UTC) | 2018-06-04 03:04:16 :: administrator -> marcelo.pazos@inpe.br :: 2014 |
| 3. Content and structure | |
| Is the master or a copy? | is the master |
| Content Stage | completed |
| Transferable | 1 |
| Content Type | External Contribution |
| Version Type | publisher |
| Keywords | BET adsorption isotherms carbon nanotube nanocomposites cell viability Fourier transform infra reds graphene oxides green fluorescent protein high-resolution scanning electron microscopies transfection efficiency carbon nanotubes cell culture Cells Composite materials DNA Fluorescence Gene transfer Genes Graphene Nanocomposites Particle size analysis Plasma etching Plasmas Scanning electron microscopy Zeta potential Molecular biology |
| Abstract | Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also known to attach oxygen content groups on the MWCNT surfaces, changing their hydrophobicity. The nanocomposite was characterised by high resolution scanning electron microscopy; energy-dispersive X-ray, Fourier transform infrared and Raman spectroscopies, as well as zeta potential and particle size analyses using dynamic light scattering. BET adsorption isotherms showed the GCN to have an effective surface area of 38.5 m2/g. The GCN and pIRES plasmid conjugated with the GFP gene, forming À-stacking when dispersed in water by magnetic stirring, resulting in a helical wrap. The measured zeta potential confirmed that the plasmid was connected to the nanocomposite. The NIH-3T3 and NG97 cell lines could phagocytize this wrap. The gene transfection was characterised by fluorescent protein produced in the cells and pictured by fluorescent microscopy. Before application, we studied GCN cell viability in NIH-3T3 and NG97 line cells using both MTT and Neutral Red uptake assays. Our results suggest that GCN has moderate stability behaviour as colloid solution and has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity and good transfection efficiency. |
| Area | FISMAT |
| Arrangement | Graphene and carbon... |
| doc Directory Content | access |
| source Directory Content | there are no files |
| agreement Directory Content | there are no files |
| 4. Conditions of access and use | |
| Language | en |
| Target File | 1-s2.0-S0928493114001180-main.pdf |
| User Group | administrator marcelo.pazos@inpe.br |
| Reader Group | administrator marcelo.pazos@inpe.br |
| Visibility | shown |
| Archiving Policy | denypublisher denyfinaldraft24 |
| Read Permission | deny from all and allow from 150.163 |
| Update Permission | not transferred |
| 5. Allied materials | |
| Mirror Repository | iconet.com.br/banon/2006/11.26.21.31 |
| Next Higher Units | 8JMKD3MGPCW/3ESR3H2 |
| URL (untrusted data) | http://dx.doi.org/10.1016/j.msec.2014.03.002 |
| Dissemination | WEBSCI; PORTALCAPES; SCOPUS. |
| Host Collection | sid.inpe.br/mtc-m21b/2013/09.26.14.25.20 |
| 6. Notes | |
| Empty Fields | alternatejournal archivist callnumber copyholder copyright creatorhistory descriptionlevel format isbn lineage mark nextedition notes orcid parameterlist parentrepositories previousedition previouslowerunit progress rightsholder schedulinginformation secondarydate secondarykey session shorttitle sponsor subject tertiarymark tertiarytype |
| 7. Description control | |
| e-Mail (login) | marcelo.pazos@inpe.br |
| update | |