| 1. Identity statement | ||||
| Reference Type | Journal Article | |||
| Site | mtc-m21d.sid.inpe.br | |||
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S | |||
| Identifier | 8JMKD3MGP3W34T/4527FL5 | |||
| Repository | sid.inpe.br/mtc-m21d/2021/07.02.13.08 (restricted access) | |||
| Last Update | 2021:07.02.13.08.53 (UTC) simone | |||
| Metadata Repository | sid.inpe.br/mtc-m21d/2021/07.02.13.08.53 | |||
| Metadata Last Update | 2022:04.03.23.14.01 (UTC) administrator | |||
| DOI | 10.3390/nano11061393 | |||
| ISSN | 2079-4991 | |||
| Citation Key | HurtadoHCQSDSTBTOCT:2021:CyAnAc | |||
| Title | Diamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity  | |||
| Year | 2021 | |||
| Month | June | |||
| Access Date | 2024, May 09 | |||
| Type of Work | journal article | |||
| Secondary Type | PRE PI | |||
| Number of Files | 1 | |||
| Size | 3423 KiB | |||
| 2. Context | ||||
| Author | 1 Hurtado, Carolina Ramos 2 Hurtado, Gabriela Ramos 3 Cena, Gabrielle Lupeti de 4 Queiroz, Rafaela Campos 5 Silva, Alexandre Vieira 6 Diniz, Milton Faria 7 Santos, Verônica Ribeiro dos 8 Trava-Airoldi, Vladimir Jesus 9 Baptista, Maurício da Silva 10 Tsolekile, Ncediwe 11 Oluwafemi, Oluwatobi Samuel 12 Conceição, Kátia 13 Tada, Dayane Batista | |||
| ORCID | 1 0000-0001-9357-6305 | |||
| Group | 1 2 3 4 5 6 7 8 COPDT-CGIP-INPE-MCTI-GOV-BR | |||
| Affiliation | 1 Instituto Federal de São Paulo (IFSP) 2 Universidade Estadual Paulista (UNESP) 3 Universidade Federal de São Paulo (UNIFESP) 4 Instituto Federal de São Paulo (IFSP) 5 6 Helmholtz Zentrum München 7 Universidade Federal de São Paulo (UNIFESP) 8 Instituto Nacional de Pesquisas Espaciais (INPE) 9 Universidade de São Paulo (USP) 10 University of Johannesburg 11 University of Johannesburg 12 Universidade Federal de São Paulo (UNIFESP) 13 Universidade Federal de São Paulo (UNIFESP) | |||
| Author e-Mail Address | 1 carolina.hurtado@ifsp.edu.br 2 gabriela.hurtado@ict.unesp.br 3 gabrielle.lupeti@unifesp.br 4 rafaelacamposqueiroz003@gmail.com 5 silva.alexandre.v@gmail.com 6 mfariadiniz@gmail.com 7 veronica.ribeiro@unifesp.br 8 vladimirjta@gmail.com 9 baptista@iq.usp.br 10 nncediwe@gmail.com 11 ooluwatobi@uj.ac.za 12 katia.conceicao@unifesp.br 13 d.tada@unifesp.br | |||
| Journal | Nanomaterials | |||
| Volume | 11 | |||
| Number | 6 | |||
| Pages | e1393 | |||
| History (UTC) | 2021-07-02 13:10:55 :: simone -> administrator :: 2021 2022-04-03 23:14:01 :: administrator -> simone :: 2021 | |||
| 3. Content and structure | ||||
| Is the master or a copy? | is the master | |||
| Content Stage | completed | |||
| Transferable | 1 | |||
| Content Type | External Contribution | |||
| Version Type | publisher | |||
| Keywords | Drug-resistance Nanoconjugate Photodynamic antimicrobial therapy (aPDT) Photosensitizers (PS) | |||
| Abstract | Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (024 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm2 ) performed right after the addition of ND-mTHPP (100 µg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT). | |||
| Area | FISMAT | |||
| doc Directory Content | access | |||
| source Directory Content | there are no files | |||
| agreement Directory Content |
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| 4. Conditions of access and use | ||||
| Language | en | |||
| Target File | hurtado-diamond.pdf | |||
| User Group | simone | |||
| Reader Group | administrator simone | |||
| Visibility | shown | |||
| Read Permission | deny from all and allow from 150.163 | |||
| Update Permission | not transferred | |||
| 5. Allied materials | ||||
| Next Higher Units | 8JMKD3MGPCW/46KUES5 | |||
| Citing Item List | sid.inpe.br/bibdigital/2022/04.03.23.11 1 | |||
| Dissemination | WEBSCI; PORTALCAPES; SCOPUS. | |||
| Host Collection | urlib.net/www/2021/06.04.03.40 | |||
| 6. Notes | ||||
| Empty Fields | alternatejournal archivingpolicy archivist callnumber copyholder copyright creatorhistory descriptionlevel e-mailaddress format isbn label lineage mark mirrorrepository nextedition notes parameterlist parentrepositories previousedition previouslowerunit progress project resumeid rightsholder schedulinginformation secondarydate secondarykey secondarymark session shorttitle sponsor subject tertiarymark tertiarytype url | |||
| 7. Description control | ||||
| e-Mail (login) | simone | |||
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